Murine ocular lymphoma
We have successfully inoculated a murine lymphoma cell line into the vitreous of adult BALB/c mice (Chan et al. Invest Ophthalmol Vis Sci 2005;46:415-9). Within 2-4 weeks tumor cells from the vitreous migrate through the retina and gather between the RPE cell and neuroretina. These lymphoma cells produce high levels of IL-10 and IFN-g transcripts. A high level of IL-10 is also found in the vitreous. This model mimics human primary intraocular lymphoma. Currently we are also developing a murine B-cell lymphoma model by intraocular inoculation of Burkitts lymphoma into SCID mice.
|Normal eye (left) and poor red reflex (right) in an eye injected with Rev-2-T-6 cells, a murine T-lymphoma cell line.|
|Rev-2-T-6 cells in the vitreous and both chambers (top); large Rev-2-T-6 cells (*) localized in the subretinal space above the choroid beneath the retina (R) and in the vitreous above the retina (bottom).